Augmented acetylcholine‐induced, Rho‐mediated Ca2+ sensitization of bronchial smooth muscle contraction in antigen‐induced airway hyperresponsive rats
- 1 June 1999
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 127 (3) , 597-600
- https://doi.org/10.1038/sj.bjp.0702585
Abstract
Treatment with acetylcholine (ACh) of a beta-escin-permeabilized intrapulmonary bronchial smooth muscle of the rat induced force when the Ca2+ concentration was clamped at 1 microM. The ACh-induced Ca2+ sensitization of myofilaments was significantly greater in antigen-induced airway hyperresponsive rats than in control rats. The ACh-induced Ca2+ sensitization was completely blocked by treatment with Clostridium botulinum C3 exoenzyme, an inactivator of Rho family of proteins. Moreover, the protein level of RhoA in the intrapulmonary bronchi was significantly increased in the airway hyperresponsive rats. Thus, increased airway smooth muscle contractility observed in asthmatics may be related to augmented agonist-induced, Rho-mediated Ca2+ sensitization of myofilaments.Keywords
This publication has 18 references indexed in Scilit:
- Role of G proteins in agonist-induced Ca2+sensitization of tracheal smooth muscleAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 1998
- Involvement of heterotrimeric GTP-binding protein and rho protein, but not protein kinase C, in agonist-induced Ca2+ sensitization of skinned muscle of guinea pig vas deferens.1995
- Alteration in Ca2+ availability involved in antigen-induced airway hyperresponsiveness in ratsEuropean Journal of Pharmacology, 1995
- Alteration of G protein levels in antigen-challenged guinea pigs.1994
- Augmented agonist-induced Ca(2+)-sensitization of coronary artery contraction in genetically hypertensive rats. Evidence for altered signal transduction in the coronary smooth muscle cells.Journal of Clinical Investigation, 1994
- Different pathways of calcium sensitization activated by receptor agonists and phorbol esters in vascular smooth muscleBritish Journal of Pharmacology, 1993
- Elevated lung G protein levels and muscarinic receptor affinity in a mouse model of airway hyperreactivityAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 1993
- Repeated Antigenic Challenge-Induced Airway Hyperresponsiveness and Airway Inflammation in Actively Sensitized RatsThe Japanese Journal of Pharmacology, 1993
- Changes in cytosolic Ca2+ and contraction induced by various stimulants and relaxants in canine tracheal smooth musclePflügers Archiv - European Journal of Physiology, 1990
- Comparison of in vivo airway responsiveness and in vitro smooth muscle sensitivity to methacholine in man.Thorax, 1984