Transposition of Tn951 (Tnlac) and Cointegrate Formation are Thermosensitive Processes
- 1 March 1980
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 117 (1) , 243-247
- https://doi.org/10.1099/00221287-117-1-243
Abstract
The frequency of transposition of Tnlac to pGC200, an IncFII R plasmid, increased during the storage of the host [Escherichia coli] strain. This result is explained by the fact that the transpositional event is temperature-dependent: it occurred readily when the host strain was grown at 30.degree. C but it was nearly undetectable when the host strain was grown and kept at 37.degree. C. Fusions between 2 different plasmids carrying Tnlac with pGC200 were also thermosensitive, suggesting a relation between cointegrate formation and transposition. Lactose did not influence the frequency of transposition of Tnlac.This publication has 6 references indexed in Scilit:
- Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements.Proceedings of the National Academy of Sciences, 1979
- Multiple integration sites for the lactose transposon Tn951 on plasmid RP1 and establishment of a coordinate system for Tn951Molecular Genetics and Genomics, 1979
- Tn951: A new transposon carrying a lactose operonMolecular Genetics and Genomics, 1978
- Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 elementJournal of Bacteriology, 1977
- Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842Journal of Bacteriology, 1976
- EPISOMIC ELEMENT IN A STRAIN OF SALMONELLA TYPHOSAJournal of Bacteriology, 1962