Formation of 22 and 24 carbon 6‐desaturated fatty acids from exogenous deuterated arachidonic acid is activated in THP‐1 cells at high substrate concentrations
Open Access
- 2 May 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 343 (3) , 195-199
- https://doi.org/10.1016/0014-5793(94)80554-7
Abstract
Deuterated arachidonic acid (AA, [2H820 :4 n‐6) 1–25 μM, is converted to other fatty acids, as evaluated by gas chromatography‐mass spectrometry, in THP‐1 cells. The major products, in the 1 to 10 μM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 μM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[2H0] ratios higher than in AA. At high AA concentration preferential conversion to elongated fatty acids with 5 unsaturations, through a 6 desaturase takes place and the 4‐desaturated 22:5 appears to be formed through β‐oxidation of 24:5.Keywords
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