Studies of the Synthetic Model Enzyme. The Synthesis of Cyclo(His–Glu–Cys-d-Phe–Gly)2 as the Esterase Model
- 1 June 1973
- journal article
- Published by Oxford University Press (OUP) in Bulletin of the Chemical Society of Japan
- Vol. 46 (6) , 1811-1816
- https://doi.org/10.1246/bcsj.46.1811
Abstract
To study the mechanism of enzyme action, cyclo(His–Glu–Cys-d-Phe–Gly)2 was prepared as an esterase model from the corresponding linear decapeptide by the azide methods. The esterase-like activity of this cyclic decapeptide in reaction to p-nitrophenyl acetate was three times greater than that of the linear decapeptide, the curve of the catalytic coefficient versus the different pH is of a bell type, and the optimum pH was recorded at about 7.6. The kinetics of the hydrolysis obeyed Michaelis-Menten’s typical equation.Keywords
This publication has 7 references indexed in Scilit:
- Thiolysis of dinitrophenylimidazoles and its use during synthesis of histidine peptidesBiochemistry, 1970
- Use of Nim-dinitrophenylhistidine in the solid-phase synthesis of the tricosapeptides 124-146 of human hemoglobin .beta. chainBiochemistry, 1969
- Synthese und Verwendung von tert.‐Butyloxycarbonylfluorid. und anderen Fluorkohlensäureestern zur Darstellung säurelabiler Urethan‐Derivate von AminosäurenEuropean Journal of Organic Chemistry, 1968
- Verbesserte Synthese von tert.‐Butyloxycarbonyl‐aminosäuren durch pH‐Stat‐ReaktionEuropean Journal of Organic Chemistry, 1967
- Synthetic Peptide Models of Enzyme Active Sites. II. L-Threonyl-L-alanyl-L-seryl-Lhistidyl-L-aspartic Acid, an Active Esterase ModelJournal of the American Chemical Society, 1964
- Synthetic histidine-containing polypeptides as catalysts for the hydrolysis of p-nitrophenyl acetateArchives of Biochemistry and Biophysics, 1960
- Tissue sulfhydryl groupsArchives of Biochemistry and Biophysics, 1959