Reduction of renin-release by puromycin

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Abstract
Puromycin infused into a renal artery (10–125 μg/kg/min for 20 min) of anaesthetized dogs consistently reduced renin activity in samples of renal venous blood when kidneys were perfused at lowered pressures. It did not affect the smaller amounts of renin present in the renal venous effluent from kidneys perfused at normal pressures. Since renin is a protein and puromycin is known to inhibit protein synthesis in vitro, it seemed logical to attribute reduction of renin-release by puromycin to inhibition of renin synthesis. But because of the early reduction of renin-release in most kidneys perfused at lowered pressures, it is probable that another mechanism is involved. However, puromycin had no effect in vitro either on the amount of angiotensin formed when it was added to incubation mixtures containing known amounts of dog renin and its substrate, or on the appearance of renin from isolated slices of dogs' kidney cortex incubated in Kreb's buffer solution.

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