A photo-CIDNP study of the interaction of oligonucleotides with gene-5 protein of bacteriophage M13.
- 1 November 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (11) , 5281-5285
- https://doi.org/10.1073/pnas.75.11.5281
Abstract
Photo-CIDNP effects (chemically induced dynamic nuclear polarization) effects can be generated in the 360 MHz proton NMR spectrum of gene-5 protein from bacteriophage M13. This technique is used to determine the number of tyrosyl residues at the surface of the protein and to assign the resonances from the 3,5-ring protons of these residues. The DNA-binding site of the protein is investigated by formation of complexes with oligonucleotides. Complex formation leads to shifting and/or quenching of the photo-CIDNP emission signals of the surface tyrosines, implying that they are involved in DNA-protein interaction. These experiments are complemented by studying the complex formation of Lys-Tyr-Lys to poly(A).This publication has 14 references indexed in Scilit:
- Laser photo-CIDNP as a surface probe for proteins in solutionNature, 1978
- Studies on DNA UnwindingEuropean Journal of Biochemistry, 1977
- Structure of gene 5 protein-oligodeoxynucleotide complexes as determined by proton, 19F, and 31P nuclear magnetic resonanceBiochemistry, 1976
- Gene 5 protein of bacteriophage fd: A dimer which interacts Co-operatively with DNAJournal of Molecular Biology, 1976
- Interactions of aromatic residues of proteins with nucleic acids. Fluorescence studies of the binding of oligopeptides containing tryptophan and tyrosine residues to polynucleotidesBiochemistry, 1975
- The Single-Stranded DNA PhagesCRC Critical Reviews in Microbiology, 1975
- The amino acid sequence of gene 5 protein of bacteriophage M 13Biochemical and Biophysical Research Communications, 1974
- Circular dichroism and ultraviolet absorption of a deoxyribonucleic acid binding protein of filamentous bacteriophageBiochemistry, 1973
- Interaction of oligopeptides containing aromatic amino acids with nucleic acids. Fluorescence and proton magnetic resonance studiesFEBS Letters, 1972
- Isolation and characterization of gene 5 protein of filamentous bacterial virusesJournal of Molecular Biology, 1972