Serum profiles of insulin‐like growth factors and their binding proteins in adults with growth hormone receptor deficiency treated with insulin like growth factor I

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Abstract
Six adult patients with growth hormone receptor deficiency (GHRD) (2 men, 4 women) with an identical defect in the growth hormone receptor (GHR) gene, were treated with recombinant human insulin‐like growth factor I (IGF‐I), 40 μgikg S.C. twice daily, for 7 days. Serum concentrations of IGF peptide and IGF binding protein‐3 (IGFBP‐3) were measured by specific radioimmunoassays; serum IGFBPs were also measured by Western ligand blotting. The size distribution of both IGF‐I and IGF‐II was measured in serum following size‐exclusion fast‐performance liquid chromatography. IGF‐I treatment resulted in a normalization of serum IGF‐I levels on days 1–7 of treatment and a decrease in serum IGF‐II levels. The fall in IGF‐II levels and the simultaneous rise in IGF‐I levels, however, resulted in an unchanged total serum IGF level. The low IGFBP‐3 values did not significantly change during treatment, whereas there was a slight increase in IGFBP‐2 levels. Preliminary analysis of size‐fractionated sera suggested an increase in IGF‐I levels in the 40 and 150 kDa regions at the expense of IGF‐II levels. The results suggest that despite the failure of IGF‐I treatment to increase IGFBPs significantly, serum IGFBP concentrations were sufficient to maintain normal levels of IGF‐I. 0 Laron syndrome, growth hormone receptor deficiency, insulin‐like growth factors, insulin‐like growth factor binding protein

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