Inhibition of membrane Ca2+-ATPase of Saccharomyces cerevisiae by mating pheromone α-factor in vitro
- 1 January 1991
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 137 (1) , 1-4
- https://doi.org/10.1099/00221287-137-1-1
Abstract
Summary: Plasma membrane Ca2+-ATPase of Saccharomyces cerevisiae was solubilized and partially purified by calmodulin-affinity chromatography. The activity of Ca2+-ATPase isolated from MATa cells was inhibited by physiological concentrations of the mating pheromone α-factor in a dose-dependent manner. The enzyme prepared from a receptor-deficient sterile mutant cells (Δste2) was similarly inhibited by α-factor, but the enzyme from MATα cells was resistant to the mating pheromone. We suggest that the inhibition may be involved in the α-factor-induced increase of Ca2+ uptake reaction of MATa cells.Keywords
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