Influenza virus M2 protein modifies membrane permeability in E. coli cells
- 2 May 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 343 (3) , 242-246
- https://doi.org/10.1016/0014-5793(94)80564-4
Abstract
The M2 protein of influenza virus is an integral membrane protein with ion channel activity. This protein has been expressed in E. coli cells in an inducible manner. Expression of the M2 protein causes rapid lysis of BL21(DE3) pLysS E. coli cells upon induction with IPTG. M2 protein increases membrane permeability to a number of hydrophylic molecules, such as ONPG, uridine or impermeant translation inhibitors. The behaviour of M2 in bacteria resembles that of other viral proteins, such as poliovirus 3A and Semliki Forest virus 6K.Keywords
This publication has 34 references indexed in Scilit:
- Inducible expression of a toxic poliovirus membrane protein in Escherichia coli: Comparative studies using different expression systems based on T7 promotersBiochemical and Biophysical Research Communications, 1992
- Cloning and inducible synthesis of poliovirus nonstructural proteinsGene, 1992
- Influenza virus M2 protein has ion channel activityPublished by Elsevier ,1992
- The M2 protein of influenza A virus is acylatedJournal of General Virology, 1991
- The Specific Inhibition of Influenza A Virus Maturation by Amantadine: An Electron Microscopic ExaminationJournal of General Virology, 1991
- Modification of membrane permeability by animal virusesPharmacology & Therapeutics, 1989
- Changes in paracellular and cellular ionic permeabilities of monolayers of MDCK cells infected with influenza or vesicular stomatitis virusesThe Journal of Membrane Biology, 1984
- Permeabilization of cells during animal virus infectionPharmacology & Therapeutics, 1983
- Membrane leakiness after viral infection and a new approach to the development of antiviral agentsNature, 1978
- The inhibition of cell functions after viral infection A proposed general mechanismFEBS Letters, 1977