Fibroblast‐dependent growth of mouse mast cells in vitro: Duplication of mast cell depletion in mutant mice of w/wv genotype
- 1 January 1988
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 134 (1) , 78-84
- https://doi.org/10.1002/jcp.1041340109
Abstract
In spite of the apparent depletion of mast cells in tissues of mutant mice of W/Wv genotype, cells with many features of mast cells do develop when bone marrow cells of W/Wv mice are cultured in the presence of pokeweed mitogen‐stimulated spleen cell‐conditioned medium (PWM‐SCM). In order to resolve this discrepancy and facilitate the analysis of the W mutation, we attempted to establish an in vitro system in which the in vivo defect of W/Wv mice can be reproduced. Cultured mast cells (CMC) were developed from bone marrow cells of either W/Wv or congenic +/+ mice, and then co‐cultured with NIH/3T3 mouse fibroblasts in media supplemented only with fetal calf serum (i.e., in the absence of PWM‐SCM). Under this condition, CMC from +/+ mice continued to divide and were maintained for more than 4 weeks. The supportive effect of NIH/3T3 cells required close‐range interactions with CMC and was not due to synthesis of the known mast cell growth factors, interleukins 3 and 4. By contrast, CMC from W/Wv mice were not maintained, and the number of mast cells remaining after 4 weeks of co‐culture was only 1% of the normal +/+ counterparts. Thus, the humoral factor‐independent and cell contact‐dependent system presented here revealed the intrinsic defects in growth and differentiation of CMC derived from W/Wv mice and might be useful for biochemical and molecular analysis of the gene product(s) encoded at the W locus.Keywords
This publication has 38 references indexed in Scilit:
- Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.The Journal of Experimental Medicine, 1985
- Neoplastic transformation of mast cells by Abelson-MuLV: abrogation of IL-3 dependence by a nonautocrine mechanismCell, 1985
- Malignant transformation of a growth factor-dependent myeloid cell line by Abelson virus without evidence of an autocrine mechanismCell, 1985
- Proliferation and differentiation in culture of mast cell progenitors derived from mast cell‐deficient mice of genotype W/WvJournal of Cellular Physiology, 1985
- Differentiation Processes of Connective Tissue Mast Cells in Living MiceInternational Archives of Allergy and Immunology, 1985
- Molecular cloning of cDNA for murine interleukin-3Nature, 1984
- A hemopoietic cell line dependent upon a factor in pokeweed mitogen-stimulated spleen cell conditioning mediumJournal of Cellular Physiology, 1980
- In vitro duplication and ‘cure’ of haemopoietic defects in genetically anaemic miceNature, 1977
- Mast cells in the skin of normal, hairless and athymic miceCellular and Molecular Life Sciences, 1976
- Berberine sulphate binding to mast cell polyanions: A cytofluorometric method for the quantitation of heparinHistochemistry and Cell Biology, 1974