Catalysis by hog‐kidney aminoacylase does not involve a covalent intermediate
Open Access
- 1 October 1986
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 160 (2) , 327-332
- https://doi.org/10.1111/j.1432-1033.1986.tb09975.x
Abstract
Hog kidney aminoacylase (N‐acylamino acid amidohydrolase; acylase I) is shown to catalyze the exchange of acetate oxygens with water at a significant rate only when alanine is present simultaneously. These studies, conducted using the 18O‐isotope induced shift on 13C‐NMR spectra, provide evidence in favor of a linear kinetic mechanism as opposed to a ‘ping‐pong’ double‐displacement mechanism. At pH values above neutrality, aminoacylase I also catalyzes the exchange of alanine oxygens with those of water. Ionic strength and pH effects on the kinetics of aminoacylase I are examined and the results are interpreted in terms of a model of the enzyme active site.Keywords
This publication has 23 references indexed in Scilit:
- Oxygen-18 isotope effect in carbon-13 nuclear magnetic resonance spectroscopy. 7. Hydrolysis of 2,2-dimethyloxirane in dilute acid and by microsomal epoxide hydrataseJournal of the American Chemical Society, 1983
- Kinetics of oxygen exchange at the anomeric carbon atom of D-glucose and D-erythrose using the oxygen-18 isotope effect in carbon-13 nuclear magnetic resonance spectroscopyBiochemistry, 1982
- Direct transfer of one-carbon units in the transformylations of de novo purine biosynthesisBiochemistry, 1982
- A kinetic study of hog kidney aminoacylaseBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- Oxygen-18 isotope effect in carbon-13 nuclear magnetic resonance spectroscopy. 4. Oxygen exchange of [1-13C,18O2]acetic acid in dilute acidJournal of the American Chemical Society, 1981
- An oxygen-18 isotope shift upon carbon-13 NMR spectra and its application to the study of oxygen exchange kineticsJournal of the American Chemical Society, 1979
- Studies of the pH dependence of carbon-13 shifts and carbon-carbon coupling constants of [U-13C]aspartic and -glutamic acidsJournal of the American Chemical Society, 1978
- The pH-Dependence of the Peptidase Activity of AminoacylaseHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- Isotope effects on the basicity of 2-nitrophenoxide, 2,4-dinitrophenoxide, hydroxide, and imidazole in protium oxide-deuterium oxide mixturesJournal of the American Chemical Society, 1967
- Synthesis of Glycyl and Alanyl Chlorides1Journal of the American Chemical Society, 1954