Identification of 4‐chloroindole‐3‐acetic acid and indole‐3‐aldehyde in seeds of Pinus sylvestris

Abstract
4‐Chloroindole‐3‐acetic acid (4‐Cl‐IAA) and indole‐3‐aldehyde (IAId) have been characterized as endogenous constituents in seeds of Pinus sylvestris L. by gas chromatography‐mass spectrometry. Quantitative estimates indicate that immature seeds contained 640 pg 4‐Cl‐IAA (g fresh weight)‐1 while mature seeds contained 340 pg (g dry weight)‐1. 4‐Cl‐IAA could not be detected in seeds five days after germination. The content of IAld increased from 127 pg (g dry weight)‐1 in mature seeds to 315 pg (g dry weight)‐1 after five days of germination.