SEROTYPING OF PSEUDOMONAS-AERUGINOSA ISOLATED FROM CLINICAL SPECIMENS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 47  (1) , 1-7
Abstract
Serotyping of 168 clinical isolates by the slide agglutination test using TIBS (Toshiba Institute of Biological Science) serotyping sera, which were prepared from Homma''s serotype strains at the request of P. aeruginosa Serotype Committee in Japan, was performed and the results were compared with serotyping using IMSUT (Institute of Medical Science, University of Tokyo) serotyping sera by the tube agglutination test. When heat-killed antigen from clinical isolates were used, both sets of serotyping sera showed similar results and the slide agglutination test using TIBS serotyping sera could be substituted for the tube agglutination test using IMSUT sera. Serotyping by the slide agglutination test with TIBS serotyping sera could be carried out using live bacteria. However, in serotyping with live bacteria, there is some difficulty in observing the agglutination time. To solve this problem positive agglutination should be determined within 60 s. Serotyping was also performed using Difco serotyping sera, and the results were compared with those obtained with serotyping sera prepared with Homma''s serotype strains. In serotyping of clinical isolates using Difco serotyping sera more cross reaction occurred than in serotyping with sera obtained from Homma''s serotype strains.