Convenient purification of human spleen fibrinolytic proteinase (SFP) and human leucocyte elastase by affinity chromatography.
- 1 January 1982
- journal article
- letter
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 30 (4) , 1528-1530
- https://doi.org/10.1248/cpb.30.1528
Abstract
A peptide inhibitor, Suc-L-Tyr-D-Leu-D-Val-pNA, for human spleen fibrinolytic proteinase (SFP) and for human leucocyte elastase was coupled with AH Sepharose 4B using water soluble carbodiimide. A 116-fold purification of the crude extract of SFP and a 59-fold purification of the partially purified leucocyte elastase were achieved by (Suc-L-Tyr-D-Leu-D-Val-pNA)-AH Sepharose 4B affinity chromatography.Keywords
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