A precursor for a small stable RNA (10Sa RNA) ofEscherichia coli
- 1 June 1989
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 217 (2-3) , 499-504
- https://doi.org/10.1007/bf02464923
Abstract
Strains carrying plasmids that code for 10Sa RNA synthesize a larger molecule when the RNA processing enzyme RNase E is inactivated. The T1 fingerprint of 10Sa RNA and the larger molecule is very similar, but the latter contains additional oligonucleotides. We show that the larger RNA is converted to the smaller, mature RNA. The precursor molecule starts with an adenosine triphosphate and is therefore a primary transcript. RNase E is not the enzyme that processes p10Sa (precursor 10Sa) RNA into 10Sa RNA. The cell extract contains an activity that carries out this conversion. This activity requires the dication Mn2+.This publication has 32 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Initiation, processing and termination of ribosomal RNA from a hybrid 5 S ribosomal RNA gene in a plasmidJournal of Molecular Biology, 1983
- A small RNA that complements mutants in the RNA processing enzyme ribonuclease PJournal of Molecular Biology, 1982
- A possible complex containing RNA processing enzymesBiochemical and Biophysical Research Communications, 1982
- Processing of bacteriophage T4 transfer RNAs structural analysis and In Vitro processing of precursors that accumulate in RNase E− strainsJournal of Molecular Biology, 1982
- Structural characterization and in vitro processing of Escherichia coli ribosomal RNA transcripts containing 5′ triphosphates, leader sequences, 16 S rRNA, and spacer tRNAsJournal of Molecular Biology, 1980
- Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coliCell, 1978
- Separation of 32P‐labelled ribonucleic acid components. The use of polyethylenimine‐cellulose (TLC) as a second dimension in separating oligoribonucleotides of ‘4.5 S’ and 5 S from E. coliFEBS Letters, 1971
- Another species of ribonucleic acid in Escherichia coliJournal of Molecular Biology, 1969
- Fractionation of 32P-labelled ribonucleic acids on polyacrylamide gels and their characterization by fingerprintingJournal of Molecular Biology, 1967