Liquid Chromatographic Identification of Urinary Catecholamine Metabolites Adsorbed on Alumina

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Abstract
Human urinary catecholamine metabolites adsorbed on alumina was identified by high-performance liquid chromatography with electrochemical detection. Since we found that some catecholamine metabolites interfered with the quantitative determination of other catecholamines such as dopamine, norepinephrine, and epinephrine in human urine samples by the liquid chromatographic method combined with the so-called alumina pretreatment procedure. It was found that alumina could adsorb urinary unidentified electroactive components as well as catecholamines. Some of the unidentified components were found to be uric acid, ascorbic acid, 3,4-dihydroxymandelic acid, 3,4-dihydroxyphenylglycol, 3-methoxy-4-hydroxymandelic acid, and 3,4-dihydroxyphenylacetic acid. On the basis of the above information, we have developed a method for the determination of catecholamines in human urine samples by high-performance liquid chromatography with electrochemical detection.

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