Meristem Tip Culture inLolium multiflorum
- 1 October 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 26 (5) , 731-736
- https://doi.org/10.1093/jxb/26.5.731
Abstract
Complete plants were regenerated from meristem tips of Lolium multiflorum. Of the three basal culture media used, Murashige and Skoog's medium gave the best results. Plantlets were produced without exogenous auxins or cytokinins, but 0.2 mg 1−1 kinetin favoured development. As the main aim in the development of this technique is the elimination of viruses, the possibilities of using 2,4.D in the culture medium and of culturing very small meristem tips for this purpose were considered.Keywords
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