Use of polylysine-coated slides in preparation of cell samples for diagnostic cytology with special reference to urine sample.

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Abstract
The standard Papanicolaou techniques of human urine cytology give rise to uneven spreading and for various reasons sometimes indifferent morphology. Filter preparations are expensive for a mass screening process and are rapidly obscured by red cells or leukocytes. The cytocentrifuge favored by some does have significant cell loss during processing and the danger of contamination of the apparatus. It gives only a small area of cells, so that a number of slides must be prepared for each sample. The polylysine method is much more consistent and gives representative cellular preparation results. Similar findings using cyst fluids, buccal smears and other cell suspensions were equally satisfactory. The essential precautions are that the polylysine solution is preserved at 4.degree. C, that the slide coatings should be prepared daily and that the area over which the cells are spread on a slide should preferably be limited.