Primary structure and expression of bovine poly(A) polymerase
- 1 September 1991
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 353 (6341) , 229-234
- https://doi.org/10.1038/353229a0
Abstract
Poly(A) polymerase has a critical role in the synthesis of messenger RNA in eukaryotic cells. The isolation and characterization of complementary DNAs encoding bovine poly(A) polymerase is described here. The predicted sequences of the mRNA and protein reveal features that provide insights into how the enzyme functions and how it might be regulated. Poly(A) polymerase expressed from a cloned cDNA is fully functional in in vitro assays, and mutational analyses have identified a putative regulatory domain that enhances, but is not essential for, activity.Keywords
This publication has 43 references indexed in Scilit:
- A beginning to the biochemistry of polyadenylationTrends in Genetics, 1988
- Accurate cleavage and polyadenylation of exogenous RNA substrateCell, 1985
- RNA sequence containing hexanucleotide AAUAAA directs efficient mRNA polyadenylation in vitro.Molecular and Cellular Biology, 1985
- Site-specific polyadenylation in a cell-free reactionCell, 1984
- Accurate and specific polyadenylation of mRNA precursors in a soluble whole-cell lysateCell, 1983
- The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAsCell, 1981
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Polyadenylic Acid Sequences in the Heterogeneous Nuclear RNA and Rapidly-Labeled Polyribosomal RNA of HeLa Cells: Possible Evidence for a Precursor RelationshipProceedings of the National Academy of Sciences, 1971
- A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites CellsProceedings of the National Academy of Sciences, 1971
- An Adenylic Acid-Rich Sequence in Messenger RNA of HeLa Cells and Its Possible Relationship to Reiterated Sites in DNAProceedings of the National Academy of Sciences, 1971