Herpes simplex virus ICP27 protein provides viral mRNAs with access to the cellular mRNA export pathway
Open Access
- 15 October 2001
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 20 (20) , 5769-5778
- https://doi.org/10.1093/emboj/20.20.5769
Abstract
The role of herpes simplex virus ICP27 protein in mRNA export is investigated by microinjection into Xenopus laevis oocytes. ICP27 dramatically stimulates the export of intronless viral mRNAs, but has no effect on the export of cellular mRNAs, U snRNAs or tRNA. Use of inhibitors shows, in contrast to previous suggestions, that ICP27 neither shuttles nor exports viral mRNA via the CRM1 pathway. Instead, ICP27‐mediated viral RNA export requires REF and TAP/NXF1, factors involved in cellular mRNA export. ICP27 binds directly to REF and complexes containing ICP27, REF and TAP are found in vitro and in virally infected cells. A mutant ICP27 that does not interact with REF is inactive in viral mRNA export. We propose that ICP27 associates with viral mRNAs and recruits TAP/NXF1 via its interaction with REF proteins, allowing the otherwise inefficiently exported viral mRNAs to access the TAP‐mediated export pathway. This represents a novel mechanism for export of viral mRNAs.Keywords
This publication has 39 references indexed in Scilit:
- Overexpression of TAP/p15 Heterodimers Bypasses Nuclear Retention and Stimulates Nuclear mRNA ExportJournal of Biological Chemistry, 2001
- Processing of α-Globin and ICP0 mRNA in Cells Infected with Herpes Simplex Virus Type 1 ICP27 MutantsJournal of Virology, 2000
- Identification of a nuclear export receptor for tRNACurrent Biology, 1998
- Immediate early protein IE63 of herpes simplex virus type 1 binds RNA directlyJournal of General Virology, 1996
- A nuclear cap binding protein complex involved in pre-mRNA splicingPublished by Elsevier ,1994
- hnRNP PROTEINS AND THE BIOGENESIS OF mRNAAnnual Review of Biochemistry, 1993
- Association of individual hnRNP proteins and snRNPs with nascent transcripts.The Journal of cell biology, 1993
- Intracellular distribution of the U1A protein depends on active transport and nuclear binding to U1 snRNA.The Journal of cell biology, 1992
- Herpes simplex virus induces a processing factor that stimulates poly(A) site usageCell, 1989
- Mutational Analysis of the Herpes Simplex Virus Type 1 Trans-inducing Factor Vmw65Journal of General Virology, 1988