Electrospray‐assisted laser desorption/ionization and tandem mass spectrometry of peptides and proteins
- 17 July 2007
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 21 (16) , 2541-2546
- https://doi.org/10.1002/rcm.3154
Abstract
We have constructed an electrospray‐assisted laser desorption/ionization (ELDI) source which utilizes a nitrogen laser pulse to desorb intact molecules from matrix‐containing sample solution droplets, followed by electrospray ionization (ESI) post‐ionization. The ELDI source is coupled to a quadrupole ion trap mass spectrometer and allows sampling under ambient conditions. Preliminary data showed that ELDI produces ESI‐like multiply charged peptides and proteins up to 29 kDa carbonic anhydrase and 66 kDa bovine albumin from single‐protein solutions, as well as from complex digest mixtures. The generated multiply charged polypeptides enable efficient tandem mass spectrometric (MS/MS)‐based peptide sequencing. ELDI‐MS/MS of protein digests and small intact proteins was performed both by collisionally activated dissociation (CAD) and by nozzle‐skimmer dissociation (NSD). ELDI‐MS/MS may be a useful tool for protein sequencing analysis and top‐down proteomics study, and may complement matrix‐assisted laser desorption/ionization (MALDI)‐based measurements. Copyright © 2007 John Wiley & Sons, Ltd.Keywords
This publication has 30 references indexed in Scilit:
- Direct characterization of intact polypeptides by matrix‐assisted laser desorption electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometryRapid Communications in Mass Spectrometry, 2007
- Gas-phase ions of solute species from charged droplets of solutionsProceedings of the National Academy of Sciences, 2007
- Top-Down ESI-ECD-FT-ICR Mass Spectrometry Localizes Noncovalent Protein-Ligand Binding SitesJournal of the American Chemical Society, 2006
- Laser desorption‐atmospheric pressure chemical ionization: a novel ion source for the direct coupling of polyacrylamide gel electrophoresis to mass spectrometryJournal of Mass Spectrometry, 2002
- Generating multiply charged protein ions via two‐step electrospray ionization mass spectrometryJournal of Mass Spectrometry, 2001
- Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic ProcessJournal of the American Chemical Society, 1998
- Primary to quaternary protein structure determination with electrospray ionization and magnetic sector mass spectrometryJournal of Mass Spectrometry, 1993
- Laser Desorption/Ionization Mass Spectrometry of Proteins of Mass 100 000 to 250 000 DaltonAngewandte Chemie International Edition in English, 1989
- Collisional effects on the charge distribution of ions from large molecules, formed by electrospray‐ionization mass spectrometryRapid Communications in Mass Spectrometry, 1988
- Protein and polymer analyses up to m/z 100 000 by laser ionization time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 1988