An 86 kDa diapause protein 1‐like protein is a component of early‐staged encapsulation‐relating proteins in coleopteran insect, Tenebrio molitor larvae1

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Abstract
Recently, we reported two novel early‐staged encapsulation‐relating proteins (56 kDa and 48 kDa ERPs) isolated from the hemolymph of coleopteran insect, Tenebrio molitor larvae [Cho et al. (1999) Eur. J. Biochem. (in press)]. Here, a cDNA clone for another early‐staged encapsulation‐relating protein (86 kDa) was isolated. We found that the 86 kDa protein shows high homology with insect diapause protein 1. The 86 kDa protein was localized in the fat body and hemolymph, but not hemocyte lysate. A significant level of 86 kDa protein was detected in pre‐pupae stage, but it decreased rapidly at late larvae and pupae, and no protein was found in embryo, early larvae and adult stages. This diapause protein 1‐like protein is likely to be a component of early‐staged encapsulation‐relating proteins in the insect cellular defense reaction.