Development of an Assay for Human Growth Hormone in Urine Using Commercially Available Reagents

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Abstract
An assay for the measurement of human growth hormone in urine (u-HGH) has been developed using commercially available reagents and its analytical variables have been investigated. The procedure is sensitive to 0·50 μU/L urine, has an interassay precision of 6·2–7·5 (CV%) over the standard curve, and shows good parallelism and recovery. Urine samples for the assay can be collected in plain plastic bottles, stored at room temperature for up to 24 h and, after adding preservative, at −20°C before assay for three months. There was a good correlation between the responses of HGH in serum and in urine during clonidine and insulin stimulation tests.