Effect of temperature on α-amylase formation and DNA replication inBacillus subtilis

Abstract
The effect of temperature on extracellular α-amylase synthesis and chromosomal and plasrnid DNA replication inBacillus subtilis A18 carrying plasmid pMI 10 was studied. The specific growth rate μ increased with elevated temperature up to 42.5°C, while the activities of α-amylase per population dry mass decreased. No obvious quantitative changes of14C-thymidine incorporation per dry mass increase and no basic differences in plasmid copy number in the range of temperatures from 25 to 40°C were found.