The Fine Structure of Yeast Regenerating Protoplasts

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Abstract
The process of regeneration of Candida utilis and Pichla polymorpha protoplasts obtained by means of an enzyme preparation from Streptomyces violaceus MR and an enzyme preparation from the snail (Helix pomatia) was followed with the electron microscope. A reticular network of fibrils making up a tube or surrounding the regenerating protoplasts was observed. The fibrils are glucan fibrils, and in C. utilis they are packed in groups of 5 or 6. P. polymorpha fibrils occurred singly. The fibrils are the framework for building the new cell wall. The relation between fibrils and plasmalemma particles is discussed.

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