A PHOTOELECTRIC METHOD FOR ESTIMATING INFLAMMATORY INTENSITY IN MICE AND ITS APPLICATION TO THE ANTI-INFLAMMATORY EVALUATION OF GLUCURONIC ACID DERIVATIVES

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Abstract
Fifty ug of extravasated dye content change in mice skin was sufficient to discriminate the differences between 2 samples in [long dash]log tx values, with 5 mice per each group. Formaldehyde and CaCl2 produced severe inflammation, and dextran, acetic acid and ethanol produced only moderate reactions; a graded dose-response relationship was determined. With histamine hydrochloride, dye leakage was provoked only in concentrations between 0. 02 to 0. 2%, and followed by a diminition of the reaction in higher concentrations. Passive cutaneous anaphylaxis induced by bovine albumin and its antiserum, made by immunizing rabbits with antigen, was recommended as the best irritant for the bioassay of anti-inflammatory drugs because of its mildness, the more general inflammatory process included. Using such irritants, the anti-inflammatory activity of well-known drugs was demonstrated. Hydrocortisone and salicylates showed almost the same effectiveness in reducing in- flammation induced by passive cutaneous anaphylaxis, formaldehyde and histamine hydrochloride, and also had a synergistic action with glucuronic acid derivatives. Aminopyrine showed activity when administered orally, but its action was inconsistent when given intravenously. Tripelennamine reduced histamine-induced inflammation remarkably when compared with the other drugs. D-Glucuronolactone, sodium D-glucuronate, D-glucaro(l[long dash]4)-lactone and 2,5-di-O-acetyl-D-glucaro-(1[long dash]4), (6[long dash]3)-dilactone were anti-inflammatory in the acute inflammation induced by passive cutaneous anaphylaxis and CaCl2, and the possible mechanism was discussed.

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