In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides.
- 1 February 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (3) , 754-757
- https://doi.org/10.1073/pnas.79.3.754
Abstract
Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 [sodium dodecyl sulfate] electrophoresis, was renatured and reassembled into short (.apprxeq. 0.05-.mu.m) .apprxeq. 10-nm-diameter filaments. The triplet polypeptides are apparently components of neural IF and the 68,000-dalton polypeptide is probably an IF structural protein.This publication has 20 references indexed in Scilit:
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