Cyclic AMP phosphodiesterases of the rat ovary. Oestrous cycle dependent activity change of high affinity form

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Abstract
In the rat ovary, from prenatal to adult stages, 2 electrophoretically distinct forms of cAMP phosphodiesterase (EC 3.1.4.17) occur. These forms were analyzed for their affinity towards cAMP in both crude and partially purified preparations. Whereas the slow electrophoretic form, which has a low affinity towards cAMP, shows a linear Lineweaver-Burk plot, the fast electromorph exhibits anomalous kinetic behavior in that cAMP affinity appears to increase with decreasing cAMP-concentration. This form shows dramatic alteration of maximum velocity during the estrous cycle, attaining maximum activity at metestrus. This rise of activity is sensitive to protein synthesis inhibition.