The mouse IFN-α (Ifa) locus: correlation of physical and linkage maps by in situ hybridization

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Abstract
The physical location of the mouse IFN-α locus (Ifa) on chromosome 4 was defined by in situ hybridization of a cloned mouse IFN-α probe to metaphase spreads in which one chromosome 4 was present as part of a single metacentric chromosome, all other chromosomes being acrocentric. (This approach greatly facilitates analysis and can be used even when it is difficult to obtain good banding.) Using unhanded chromosomes, the grains were localized over the chromosome 4 part of the metacentric, in a region 0.61 + 0.07 (SD) of the distance from the centromere to the telomere. In Giemsa-banded spreads, the majority of the grains were in the region 4C3→C6. Consideration of these results and of the known linkage maps for mouse and man indicates that the Gait – Aco-1 – Ifa syntenic group spans a distance of ∼14 cM and suggests that the same group on human 9p will also occupy a similarly sized region, with GALT proximal and IFL distal to the centromere.