Scanning electron microscopy of chromosomes of common wheat

Abstract
The higher magnifications and resolution possible with scanning electron microscopy can provide more detailed information on chromosome morphology than light microscopy. However, overlying cell debris can obscure surface details. A method is described whereby standard organic acid–alcohol fixatives and slightly modified techniques of routine plant chromosome squash preparations can be used to provide samples of mitotic and meiotic chromosomes of wheat suitable for examination by scanning electron microscopy.Key words: scanning electron microscopy, light microscopy, wheat, chromosomes.

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