ALDOSTERONE AND CORTICOSTERONE PRODUCTION BY RAT ADRENAL SLICES AS A FUNCTION OF THE TIME OF INCUBATION
- 1 January 1966
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 44 (1) , 85-89
- https://doi.org/10.1139/o66-010
Abstract
Some factors affecting steroid production by rat adrenals in vitro have been studied. Aldosterone production by normal glands increases linearly for at least 20 h. of incubation, whereas corticosterone production reaches a plateau after 4 h. Aldosterone production by adrenals from rats kept on 1% NaCl as drinking fluid for 8 days does not increase after the 2 hour of incubation. Varying the volume of Krebs-Ringer bicarbonate medium (containing a given amount of tracer 4-C14 progesterone) does not alter the endogenous production of aldosterone or corticosterone, but the specific activity of these compounds decreases as the volume of Krebs-Ringer bicarbonate increases. The significance of these findings is discussed.This publication has 0 references indexed in Scilit: