EFFECTS OF GTP ON CYCLIC-AMP CONCENTRATIONS IN INTACT EHRLICH ASCITES TUMOR-CELLS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 3  (5) , 347-354
Abstract
Experiments were conducted to test the hypothesis that intracellular concentrations of GTP regulate activity and hormonal sensitivity of adenylate cyclase in intact cells. By appropriate treatments, GTP concentrations of Ehrlich ascites tumor cells could be varied between 22-680% of control values. Cyclic[c]AMP concentrations were measured before and after addition of epinephrine in cells containing this range of GTP concentrations. Basal cAMP concentrations were unaffected by changes in GTP concentrations. In cells containing lowered concentrations of GTP, the cAMP concentration following addition of epinephrine was half that in control cells. Elevation of GTP concentrations above normal had no effect on cAMP concentrations following epinephrine treatment.

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