Cloning of the pig aminopeptidase N gene
- 17 July 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 251 (1-2) , 275-281
- https://doi.org/10.1016/0014-5793(89)81470-x
Abstract
We have isolated four λ‐phages covering the complete pig aminopeptidase N/CD 13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5′ upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF‐1/NF‐1 and AP‐2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human α1‐antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the Functional peptide.Keywords
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