The Endogenous Sulfated Pentapeptide Phytosulfokine-α Stimulates Tracheary Element Differentiation of Isolated Mesophyll Cells of Zinnia1
Open Access
- 1 August 1999
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 120 (4) , 1043-1048
- https://doi.org/10.1104/pp.120.4.1043
Abstract
Dispersed zinnia (Zinnia elegans) mesophyll cells cannot differentiate into tracheary elements (TEs) at low cell density conditions even if auxin and cytokinin are present in the medium, indicating the involvement of intercellular interactions during the initiation and/or subsequent progresses in TE differentiation. When zinnia cells were incubated at a low density (2.5 × 104 cells mL−1) in TE-inductive medium in the presence of various concentrations of phytosulfokine (PSK)-α, which was originally identified as an intercellular signal peptide involved in cell proliferation, TE differentiation was strongly stimulated in a dose-dependent fashion; more than 35% of the living cells differentiated into TEs by 5 d of culture in the presence of 10 nm PSK-α. Enzyme-linked immunosorbent assay and mass spectroscopy confirmed that cultured zinnia cells produce nanomolar levels of PSKs under inductive conditions. These results suggest that PSK-α is a factor responsible for TE differentiation of zinnia mesophyll cells.Keywords
This publication has 14 references indexed in Scilit:
- Physiological relationships between auxin, cytokinin, and a peptide growth factor, phytosulfokine-α, in stimulation of asparagus cell proliferationPlanta, 1999
- Effects of the medium ammonium-nitrate ratio on competence for asparagus cell division induced by phytosulfokine- αPlant Cell Reports, 1998
- Phytosulfokine-α, a sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high- and low-affinity binding sitesProceedings of the National Academy of Sciences, 1997
- Active Fragments and Analogs of the Plant Growth Factor, Phytosulfokine: Structure–Activity RelationshipsBiochemical and Biophysical Research Communications, 1996
- Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L.Proceedings of the National Academy of Sciences, 1996
- Tracheary Element Formation as a Model System of Cell DifferentiationPublished by Elsevier ,1992
- Characterization of Conditioning Factors that Increase Colony Formation from «Black Mexican Sweet Corn» ProtoplastsJournal of Plant Physiology, 1988
- Conditioning factor affecting growth in plant cells in culturePlant Science, 1987
- Direct Evidence for Cytodifferentiation to Tracheary Elements without Intervening Mitosis in a Culture of Single Cells Isolated from the Mesophyll of Zinnia elegansPlant Physiology, 1980
- Establishment of an Experimental System for the Study of Tracheary Element Differentiation from Single Cells Isolated from the Mesophyll of Zinnia elegansPlant Physiology, 1980