The precipitation of proteins with complex salts

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Abstract
Ionized inorganic complex-salts are useful protein precipitants. Complex anions precipitate proteins when used in solns. more acid than the isoelectric point of the protein, whereas complex cations precipitate proteins when used at a pH more alkaline than the isoelectric point of the protein; e.g., serum albumin is precipitated at pH 2 by K3[Cr(C2O4)3] and at pH 7 by [Co(NH3)6]Cl3. Quantitative detns. showed that proteins are combined in the precipitates with complex ions in stoichiometric proportions. The proteins react in acid soln. with complex anions with their "maximal acid-combining capacity"; they combine, on the other hand, with complex cations at or near pH 7 with only a fraction of their "maximal base-combining capacity.".

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