Preparation of Functionally PEGylated Gold Nanoparticles with Narrow Distribution through Autoreduction of Auric Cation by α-Biotinyl-PEG-block-[poly(2-(N,N-dimethylamino)ethyl methacrylate)]

Abstract
PEGylated gold nanoparticles with biotin moieties installed at the distal end of the PEG tethered chains were prepared by the autoreduction of HAuCl4 catalyzed by α-biotinyl-PEG-block-poly[2-(N,N-dimethylamino)ethyl methacrylate] (biotinyl-PEG/PAMA) in aqueous medium at room temperature. The size of the gold nanoparticles was controllable in a range of 6−13 nm by changing the initial Au3+/polymer ratio, while retaining their narrow size distribution. The dispersion stability of the nanoparticles in aqueous medium was extremely high even under the condition of salt concentration as high as I = 2.0. Biotinyl-PEG/PAMA-anchored gold nanoparticles underwent specific aggregation in the presence of streptavidin, revealing their promising utility as colloidal sensing systems applicable under biological condition.

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