Bacteroides gingivalis: Recognition of a 47 kDa Outer Membrane Protein by Sera from Patients with Chronic Periodontitis

Abstract
Sarcosyl-insoluble outer membrane components of Bacteroides gingivalis were separated by SDS-PAGE and electroblotted onto nitrocellulose membranes. They were then probed with sera from patients with a history of advanced periodontitis (CPITN of 4) and from a control group of subjects with little or no history of periodontitis (CPITN of 3 or less). A47 kDa antigen was recognised by sera from all fifteen patients but not by nine of ten subjects in the control group.