Interaction between Normal and Leukaemic Human Cells in Agar Culture

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Abstract
The influence of leukemic cells from 12 patients with acute leukemia on normal granulopoiesis in agar culture was investigated using leukemic cell feeder layers. Leukemic feeder cells from 7 of the 12 patients elicited no colony growth, while cells from the remaining 5 stimulated normal colony growth. In 3 of the 7 non-stimulatory patients, release of inhibitory factors from the leukemic cells seemed responsible for the effect on normal granulopoiesis, while inappropriate colony stimulating factor (CSF) production by the feeder cells could not be ruled out in the remaining 4 patients. When the leukemic cells were cultured with, as well as without, conditioned medium, cells from 5 of the patients formed clusters. Growth in these cultures did not correlate to the effect found in the feeder layer experiments.