Isolation of mutants deregulated in phosphate control of candicidin biosynthesis.

Abstract
Mutants [Streptomyces griseus] were isolated in which phosphate does not inhibit the biosynthesis of candicidin. At high phosphate concentrations, candicidin production by phosphate-deregulated mutants is still inhibited, but to a lesser extent than in the wild type. Some of these mutants are higher candicidin producers than the wild type in phosphate-supplemented medium and also in non-supplemented production medium. The high candicidin production by these mutants is due to a high specific rate of candicidin biosynthesis and an extended production phase. None of the phosphate-deregulated mutants in which uptake of [32P]phosphate was measured was a phosphate-permeability mutant.

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