Molecular mechanisms of odor sensing. V. Some biochemical characteristics of the alanineous receptor from the olfactory epithelium of the skate Dasyatis pastinaca
- 1 July 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Chemical Senses
- Vol. 5 (3) , 195-203
- https://doi.org/10.1093/chemse/5.3.195
Abstract
The membrane fraction of skale olfactory tissue was found to contain structures with high affinity to alanine (K D =0.l3.l0 −9 M). The acceptor sites were highly specific to alanine: only amino acids with molecular structures similar to that of alanine could compete with alanine successfully. Some parameters of the “alanine” receptor are evaluated. Triton X-100 has no marked effect on the binding of alanine. The isoelectric point of the receptor solubilized with Triton X-100 is 5.65, as determined by isoclectric focusing. The molecular weight of the receptor as determined by gel electrophoresis is about 120 000 D.Keywords
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