Method of Separation and Identification of Proteases by Electrophoresis on Cellulose Acetate Membrane

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Abstract
A method of separation and identification of proteases by electrophoresis on cellulose acetate membrane is presented. Proteases were separated by electrophoresis on cellulose acetate membrane. The membrane was layered onto a substrate-agar plate and incubated. The proteolysis areas were distinguished from the non-digested back-ground by direct observation or by protein staining. The electrophoretic localization of proteases tested were as follows: Trypsin (×3 crystallized): post-γ globulin fraction (1) Trypsin (commercial): α-2, β and post-γ globulin fractions (3) Chymotrypsin: post-γ globulin fraction (1) Bromelain: post-γ globulin fraction (1) Pronase: post-γ globulin fractions (3) Urokinase: diffuse digestion between α-2 and post-β globulin fractions (3) Plasminogen in serum: α-2 and β globulin fractions (2) Figures in parentheses indicate the number of lysis area.

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