Molecular cloning of a unique human T-cell leukemia virus (HTLV-IIMo).

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Abstract
Human T-cell leukemia virus IIMo (HTLV-IIMo) is a human retrovirus isolated from a patient with a T-cell hairy cell leukemia. This virus has been shown to have core protein (gag) antigens similar to, but distinct from, those of all known isolates of the prototype human T-cell leukemia virus (HTLV-I). A subgenomic clone of the HTLV-I env-pX region was used to detect and characterize HTLV-IIMo proviral sequences by performing Southern blot hybridization under conditions of low stringency. Using the HTLV-I probe, a partial integrated HTLV-IIMo provirus was cloned from a genomic library of the producer Mo cell line. These sequences could be characterized by low stringency hybridization with different subgenomic clones of HTLV-I. An HTLV-IIMo-specific subclone was made by isolating a 3.6-kilobase BamHI fragment of the partial provirus. This was used to clone 2 full-length integrated viral genomes. Using the HTLV-IIMo viral probe, these HTLV-IIMo sequences were shown tgo be unique by hybridization under stringent conditions to DNA and RNA of various infected and uninfected cell lines.