Modified box‐cox transform for modulating the dynamic range of flow cytometry data

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Abstract
We describe an algorithm, based upon Box‐Cox transformations as an alternative to nonlinear electronic amplifiers to expand or compress high‐ or low‐amplitude flow cytometer‐derived signals. If the indexing parameter λ < 1, input channels in the high‐amplitude input range are compressed in the output range as occurs when an electronic logarithmic amplifier is used. However, if λ > 1, input channels in the low‐amplitude input range are compressed in the output range as occurs when an electronic power amplifier is used. Our modified Box‐Cox transform can be implemented either during data collection or off‐line for the transformation of previously collected raw data. The transform is the equivalent of an infinite class of nonlinear amplifiers. As the transform is implemented in software, it does not suffer from many of the disadvantages of nonlinear electronic amplifiers.

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