Targeted transfection by femtosecond laser
Top Cited Papers
- 1 July 2002
- journal article
- Published by Springer Nature in Nature
- Vol. 418 (6895) , 290-291
- https://doi.org/10.1038/418290a
Abstract
The challenge for successful delivery of foreign DNA into cells in vitro, a key technique in cell and molecular biology with important biomedical implications, is to improve transfection efficiency while leaving the cell's architecture intact. Here we show that a variety of mammalian cells can be directly transfected with DNA without perturbing their structure by first creating a tiny, localized perforation in the membrane using ultrashort (femtosecond), high-intensity, near-infrared laser pulses. Not only does this superior optical technique give high transfection efficiency and cell survival, but it also allows simultaneous evaluation of the integration and expression of the introduced gene.Keywords
This publication has 10 references indexed in Scilit:
- Studying protein dynamics in living cellsNature Reviews Molecular Cell Biology, 2001
- The many ways to cross the plasma membraneProceedings of the National Academy of Sciences, 2001
- Multiphoton microscopy in life sciencesJournal of Microscopy, 2000
- Transfection of cells by immunoporationNature, 2000
- Synthetic DNA delivery systemsNature Biotechnology, 2000
- A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotesNature Biotechnology, 1999
- Long-term two-photon fluorescence imaging of mammalian embryos without compromising viabilityNature Biotechnology, 1999
- Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopyCurrent Biology, 1999
- Time‐resolved fluorescence microscopy using two‐photon excitationBioimaging, 1995
- Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane.Proceedings of the National Academy of Sciences, 1987