Comparison of Lipid Metabolism of Chicken Embryo Organs and Cells in Culture.

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Abstract
A study was made of the relative incorporation of acetate-1-C14 into lipids of chicken embryo tissues in ovo and in tissue culture. Embryonic heart, thigh, and stomach incorporated C14 mainly into phospholipids in ovo, and into neutral fat in vitro. Relative C14 content of the cholesterol esters was higher when the cells were grown in medium containing calf serum as compared with chicken serum, an effect not correlated with serum fatty acid composition or fatty acid and cholesterol content. Acetate incorporation by established cell lines (HeLa and Chang liver) resembled more closely that of chick embryo organs in ovo rather than that of chick embryo tissues in vitro.