ENZYME-LABELED ANTIBODIES: PREPARATION AND APPLICATION FOR THE LOCALIZATION OF ANTIGENS

1 December 1966

journal article
letter
Published by SAGE Publications in Journal of Histochemistry & Cytochemistry

Vol. 14 (12) , 929-931
https://doi.org/10.1177/14.12.929

Abstract

Horse-radish peroxidase labeled rabbit antibody with the tissue and after removal of the unreacted globulin by washing, the antibody-globulin bound to its antigen was localized by reacting the tissue with enzyme labeled sheep anti-rabbit gamma globulin. Enzyme labeled antibodies have distinct advantages over the fluorescent antibody method. The preparations stained with peroxidase antibody are permanent, and may be examined with ordinary light microscopes.

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