Purification of a troponin I‐like factor from pig platelet
- 21 February 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 152 (2) , 202-206
- https://doi.org/10.1016/0014-5793(83)80380-9
Abstract
A troponin I‐like factor has been purified from pig platelet by G150 Sephadex filtration of a low ionic strength extract, acidification at pH 4.2, ion exchange on DE‐52 cellulose, and affinity chromatography on calmodulin‐Sepharose. This protein (M r 17000), together with pig brain calmodulin and platelet tropomyosin, is able to participate to the reconstitution in vitro of a thin filament‐like complex which modulates with 55% calcium sensitivity 1 the platelet actin‐activated Mg2+‐dependent ATPase activity of rabbit skeletal muscle myosin.Keywords
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