Identification of a novel Rac1-interacting protein involved in membrane ruffling.
- 1 August 1996
- journal article
- Vol. 15 (15) , 3778-86
Abstract
The Rac GTP binding proteins are implicated in actin cytoskeleton-membrane interaction in mammalian cells. In fibroblast cells, Rac has been shown to mediate growth factor-induced polymerization of actin to form membrane ruffles and lamellipodia. We report here the isolation of a noval Rac1-interacting protein, POR1. POR1 binds directly to Rac1, and the interaction of POR1 with Rac1 is GTP dependent. A mutation in the Rac1 effector binding loop shown to abolish membrane ruffling also abolishes interaction with POR1. Truncated versions of POR1 inhibit the induction of membrane ruffling by an activated mutant of Rac1, V12Rac1, in quiescent rat embryonic fibroblast REF52 cells. Furthermore, POR1 synergizes with an activated mutant of Ras, V12Ras, in the induction of membrane ruffling. These results suggest a potential role for POR1 in Rac1-mediated signaling pathways.This publication has 55 references indexed in Scilit:
- The Rac and Rho pathways as a source of drug targets for Ras-mediated malignanciesCurrent Opinion in Biotechnology, 1995
- Pheromone Response in Yeast: Association of Bem1p with Proteins of the MAP Kinase Cascade and ActinScience, 1995
- Rho Family GTPases Regulate p38 Mitogen-activated Protein Kinase through the Downstream Mediator Pak1Journal of Biological Chemistry, 1995
- Identification of a Mouse p21Cdc42/Rac Activated KinasePublished by Elsevier ,1995
- Rac mediates growth factor-induced arachidonic acid releasePublished by Elsevier ,1995
- Small GTP-Binding Proteins and the Regulation of the Actin CytoskeletonAnnual Review of Cell Biology, 1994
- Identification of a novel protein with GDP dissociation inhibitor activity for the ras‐like proteins CDC42Hs and Rac 1Genes, Chromosomes and Cancer, 1993
- Oncogene ect2 is related to regulators of small GTP-binding proteinsNature, 1993
- The small GTP-binding protein rac regulates growth factor-induced membrane rufflingCell, 1992
- At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cellsJournal of Molecular Biology, 1987