Different Utilization of Deoxycytidine as DNA-Thymine in Lymphoid Tissues of the Mouse
- 1 January 1978
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 3 (3) , 255-257
- https://doi.org/10.1247/csf.3.255
Abstract
Small pieces of tissues were cut and homogenized in an appropriate volume of 2 M NaCl. Pure, highly polymerized DNA was extracted after RNase and protease treatment from the homogenate of lymphoid tissues of mouse. The extracted DNA contained no detectable RNA, and the yields ranged from 30-70% of DNA in the starting material. Using pure DNA extracted by this method, the rate of [G-3H]deoxycytidine incorporation into cytosine and thymine of DNA in thymus was different from that in mesenteric lymph nodes in mouse after adminsitration of [G-3H]deoxycytidine. The ultilization of deoxycytidine as DNA-thymine was differed between the thymus and mesenteric lymph nodes in the mouse.This publication has 1 reference indexed in Scilit:
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961