RNA-DNA hybridization analyses of tRNA 3b Val in Drosophila melanogaster

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Abstract
Transfer RNA was extracted from 50–300 mg of adult flies and specifically labeled in vitro. The level of individual isoacceptors was quantitated by efficient annealing to Drosphila tRNA genes carried on recombinant DNA plasmids immobilized on nitrocellulose filters. The level of tRNA 3b Val in the tRNA isolated from flies deficient in the major tRNA 3b Val loci has been examined. The results show that deletion of the major tRNA 3b Val loci resulted in a reduction of approximately 50% in the level of tRNA 3b Val but did not produce the Minute phenotype; furthermore the effects of deficiencies at two loci were approximately additive.